Title: Event-specific Method for the Quantification of Maize 98140 by Real-time PCR
Authors: SAVINI CristianMAZZARA MarcoBEVILACQUA A.VAN DEN EEDE Guy
Publisher: Publications Office of the European Union
Publication Year: 2011
JRC N°: JRC68064
ISBN: 978-92-79-22436-2
ISSN: 1831-9424
Other Identifiers: EUR 25129 EN
OPOCE LB-NA-25129-EN-N
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC68064
DOI: 10.2788/34828
Type: EUR - Scientific and Technical Research Reports
Abstract: The European Union Reference Laboratory for GM Food and Feed (EU-RL GMFF), established by Regulation (EC) No 1829/2003, has carried out a validation study to assess the performance of a quantitative event-specific method on the maize event 98140 (unique identifier DP-098140-6). The collaborative trial was conducted according to internationally accepted guidelines. In accordance to Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and to Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Pioneer Overseas Corporation provided the detection method and the control samples. The EU-RL GMFF prepared the validation samples [calibration samples and blind samples at unknown GM percentages(DNA/DNA)]. The results of the international collaborative trial met the European Network of GMO Laboratories (ENGL) method performance requirements (http://gmocrl.jrc.ec.europa.eu/guidancedocs.htm). The method is therefore considered applicable to the control samples provided, in accordance with the requirements of Annex I – 2.C.2 to Regulation (EC) No 641/2004.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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