MULTI RESIDUE METHOD FOR THE DETECTION OF ANTIMICROBIAL AGENTS IN DRIED DISTILLERS GRAINS BY LIQUID CHROMATOGRAPHY-HIGH RESOLUTION MASS SPECTROMETRY

To enable the detection of antimicrobial agents in Dried Distillers Grains (DDGs), a liquid chromatography-high resolution mass spectrometry (LC-HRMS) method was developed including a wide range of classes of antibiotics (authorized coccidiostats, banned coccidiostats, macrolides, tetracyclines, quinolones and sulphonamides). The compounds were extracted with a mixture of organic solvents, filtered, centrifuged and directly injected to the Orbitrap. The ionization mode was heated- Electrospray ionization (H-ESI) in positive and negative full scan mode. Analysis was performed with a high resolving power of 50.000 to achieve acceptable mass accuracy. The method proved to have acceptable accuracy and is capable to provide highly selective qualitative and quantitative analysis.

KAKLAMANOS Georgios;  VINCENT Ursula; 

2017-04-28

The Euroresidue Conference foundation

JRC69202

https://publications.jrc.ec.europa.eu/repository/handle/JRC69202,