Evaluation of plasmid and genomic DNA calibrants used for the quantification of genetically modified organisms
The reliable quantification of genetically modified organisms (GMOs) by real-time PCR requires, besides thoroughly validated quantitative detection methods, sustainable calibration systems. The latter establishes the anchor points for the measured value and the measurement unit, respectively. In this paper, the suitability of two types of DNA calibrants, i.e. plasmid DNA and genomic DNA extracted from plant leaves, for the certification of the GMO content in reference materials as copy number ratio between two targeted DNA sequences was investigated. The paper outlines, that the most suitable approach to establish a sustainable traceability chain is to fix a reference system based on plasmid DNA.
CAPRIOARA-BUDA Mihaela;
MEYER Wibke;
JEYNOV Boyan;
CORBISIER Philippe;
TRAPMANN Stefanie;
EMONS Hendrik;
2012-10-11
SPRINGER HEIDELBERG
JRC69642
1618-2642,
https://publications.jrc.ec.europa.eu/repository/handle/JRC69642,
10.1007/s00216-012-6104-6,
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