Assessment of Metabolic Stability Using the Rainbow Trout (Oncorhynchus mykiss) Liver S9 Fraction
Standard protocols are given for assessing metabolic stability in rainbow trout using the liver S9 fraction. These protocols describe the isolation of S9 fractions from trout livers, evaluation of metabolic stability using a substrate depletion approach, and expression of the result as in vivo intrinsic clearance. Additional guidance is provided on the care and handling of test animals, design and interpretation of preliminary studies, and development of analytical methods. Although initially developed to predict metabolism impacts on chemical accumulation by fish, these procedures can be used to support a broad range of scientific and risk assessment activities including evaluation of emerging chemical contaminants and improved interpretation of toxicity testing results.
JOHANNING Karla;
HANCOCK G.;
ESCHER Beate;
ADEKOLA Adelbayo;
BERNHARDT Maryjo;
COWAN-ELLSBERRY Christina;
DOMORADZKI Jean;
DYER Scott;
EICKHOFF Curtis;
EMBRY Michelle;
ERHARDT Sue;
FITZSIMMONS Patrick;
HALDER Maria;
HILL James;
HOLDEN D;
JOHNSON Rebecca;
RUTISHAUSER Sibylle;
SEGNER Helmut;
SCHULTZ Irvin;
NICHOLS John;
2013-03-05
John Wiley
JRC72001
https://publications.jrc.ec.europa.eu/repository/handle/JRC72001,
10.1002/0471140856.tx1410s53,
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