Title: Assessment of Metabolic Stability Using the Rainbow Trout (Oncorhynchus mykiss) Liver S9 Fraction
Authors: JOHANNING KarlaHANCOCK G.ESCHER BeateADEKOLA AdelbayoBERNHARDT MaryjoCOWAN-ELLSBERRY ChristinaDOMORADZKI JeanDYER ScottEICKHOFF CurtisEMBRY MichelleERHARDT SueFITZSIMMONS PatrickHALDER MariaHILL JamesHOLDEN DJOHNSON RebeccaRUTISHAUSER SibylleSEGNER HelmutSCHULTZ IrvinNICHOLS John
Citation: Current protocols in Toxicology vol. Supplement 53 p. 14.10.1-14.10.28
Publisher: John Wiley
Publication Year: 2012
JRC N°: JRC72001
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC72001
DOI: 10.1002/0471140856.tx1410s53
Type: Articles in periodicals and books
Abstract: Standard protocols are given for assessing metabolic stability in rainbow trout using the liver S9 fraction. These protocols describe the isolation of S9 fractions from trout livers, evaluation of metabolic stability using a substrate depletion approach, and expression of the result as in vivo intrinsic clearance. Additional guidance is provided on the care and handling of test animals, design and interpretation of preliminary studies, and development of analytical methods. Although initially developed to predict metabolism impacts on chemical accumulation by fish, these procedures can be used to support a broad range of scientific and risk assessment activities including evaluation of emerging chemical contaminants and improved interpretation of toxicity testing results.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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