Interaction of magnetic nanoparticles with U87MG cells studied by synchrotron radiation X-ray fluorescence techniques
Synchrotron radiation (SR) X-ray microscopy (XRM) combined with X-ray
fluorescence (XRF) microspectroscopy provides unique information that have pushed
the frontiers of biological research, particularly when investigating intracellular
mechanisms. This work reports a SR-XRF microspectroscopy investigation on the
distribution and the potential toxicity of Fe3O4 and CoFe2O4 nanoparticles (NPs) in
U87MG glioblastoma-astrocytoma cells. The U87MG cells exposed to NPs
concentrations ranging from 5 to 250 μg/mL for 24h were analyzed in order to
monitor both morphological and chemical changes. The SR-XRF maps
complemented with XRM absorption and phase contrast images have revealed
different intracellular distribution patterns for the two nanoparticles types allowing
deduce different mechanism of toxicity.
GIANONCELLI Alessandra;
MARMORATO Patrick;
PONTI Jessica;
PASCOLO Lorella;
KAULICH Burkhard;
UBOLDI Chiara;
ROSSI Francois;
MAKOVEC Darko;
KISKINOVA Maya;
CECCONE Giacomo;
2014-01-15
WILEY-BLACKWELL
JRC72236
0049-8246,
http://onlinelibrary.wiley.com/doi/10.1002/xrs.2475/abstract,
https://publications.jrc.ec.europa.eu/repository/handle/JRC72236,
10.1002/xrs.2475,
Additional supporting files
| File name | Description | File type | |
|---|
