Report on the validation of a method for determination of Ochratoxin A in Capsicum spp. (paprika and chilli)
A method validation study was conducted according to the IUPAC harmonised protocol for the determination of ochratoxin A in Capsicum spp. (paprika and chilli). The method is based on the extraction of the samples with an aqueous methanol solution, followed by immunoaffinity cleanup. The determination is carried out by reversed phase high performance liquid chromatography coupled to a fluorescence detector. The study involved 21 participants representing a cross section of research, private and official control laboratories from 14 EU Member States and Singapore.
Mean recoveries reported ranged from 83.7 to 87.5. The relative standard deviation for repeatability (RSDr) ranged from 1.7 to 14.3 %. The relative standard deviation for reproducibility (RSDR) ranged from 9.1 to 27.5 %, reflecting HorRat values from 0.4 to 1.3 according to the Horwitz function modified by Thompson. A correction for recovery with the data generated by fortification experiments further improved the reproducibility performance of the method.
The method showed acceptable within-laboratory and between-laboratory precision for each matrix, as required by current European legislation.
KUNSAGI Zoltan;
STROKA Joerg;
2012-09-17
Publications Office of the European Union
JRC73114
978-92-79-25827-5,
1831-9424,
EUR 25458 EN,
OP LA-NA-25458-EN-N,
https://publications.jrc.ec.europa.eu/repository/handle/JRC73114,
10.2787/64803,
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