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dc.contributor.authorPASCUAL GARCIA CESARen_GB
dc.contributor.authorSUMBAYEV Vadimen_GB
dc.contributor.authorGILLILAND Douglasen_GB
dc.contributor.authorYASINSKA Innaen_GB
dc.contributor.authorGIBBS Bernharden_GB
dc.contributor.authorMEHN Doraen_GB
dc.contributor.authorCALZOLAI LUIGIen_GB
dc.contributor.authorROSSI Francoisen_GB
dc.date.accessioned2016-06-30T00:27:49Z-
dc.date.available2016-06-28en_GB
dc.date.available2016-06-30T00:27:49Z-
dc.date.created2016-06-21en_GB
dc.date.issued2013en_GB
dc.date.submitted2012-07-25en_GB
dc.identifier.citationSCIENTIFIC REPORTS vol. 3en_GB
dc.identifier.issn2045-2322en_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC73187-
dc.description.abstractGold nanoparticles (AuNPs) can interfere with some of the biochemical processes of macrophage cells but the mechanisms of action of these potentially medically-relevant effects are still unclear. Here we study the fate of AuNPs interacting with cells derived from the innate immune system. To visualize AuNPs with nanometer resolution without losing sight of the whole morphology of cells we developed a convenient approach that uses electron and ion microscopy techniques. The inspection using an ion beam to selectively cut where the AuNPs were found, allows for determining their intracellular localizations. We studied the cellular uptake of AuNPs, with or without exposure of the cells to Latrunculin-A, a phagocytosis inhibitor. Results indicate a size dependence of the internalization mechanisms for THP-1 cells. The internalization of larger AuNPs was blocked in the presence of Latrunculin-A, although they could attach to the membrane. Smaller AuNPs though were not blocked by actin depending processes.en_GB
dc.description.sponsorshipJRC.I.4-Nanobiosciencesen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherNATURE PUBLISHING GROUPen_GB
dc.relation.ispartofseriesJRC73187en_GB
dc.titleMicroscopic Analysis of the Interaction of Gold Nanoparticles with Cells of the Innate Immune Systemen_GB
dc.typeArticles in periodicals and booksen_GB
dc.identifier.doi10.1038/srep01326en_GB
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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