Title: Event-specific method for the quantification of soybean MON 87705 using real-time PCR validation report
Publisher: Publications Office of the European Union
Publication Year: 2012
JRC N°: JRC74376
ISBN: 978-92-79-26278-4
ISSN: 1831-9424
Other Identifiers: EUR 25499 EN
URI: http://gmo-crl.jrc.ec.europa.eu/statusofdoss.htm
DOI: 10.2788/48383
Type: EUR - Scientific and Technical Research Reports
Abstract: The European Union Reference Laboratory for Genetically Modified Food and Feed (EU-RL GMFF), established by Regulation (EC) No 1829/2003, in collaboration with the European Network of GMO Laboratories (ENGL), has carried out a collaborative study to assess the performance of a quantitative event-specific method to detect and quantify the MON 87705 transformation event in soybean DNA (unique identifier MON-877Ø5-6). The collaborative trial was conducted according to internationally accepted guidelines (1, 2). In accordance with Regulation (EC) No 1829/2003 of 22 September 2003 “on genetically modified food and feed” and with Regulation (EC) No 641/2004 of 6 April 2004 “on detailed rules for the implementation of Regulation (EC) No 1829/2003”, Monsanto provided the detection method and the samples (genomic DNA from soybean seeds harbouring the MON 87705 event and from conventional soybean seeds). The EU-RL GMFF prepared the validation samples (calibration samples and blind samples at unknown GM percentage [DNA/DNA]). The collaborative trial involved twelve laboratories from nine European countries. The results of the international collaborative trial met the ENGL performance requirements. The method is, therefore, considered applicable to the control samples provided, in accordance with the requirements of Annex I-2.C.2 to Regulation (EC) No 641/2004. The results of the collaborative study are made publicly available at http://gmo-crl.jrc.ec.europa.eu/
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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