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Verification Report on the extraction and analysis of GM pollen DNA in honey

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Following the judgment of 06 September 2011 on GM honey by the European Court of Justice (legal case C-442-09), the European Union Reference Laboratory for Genetically Modified Food and Feed (EU-RL GMFF) established by Regulation (EC) No 1829/2003, performed an in-house study to test the extraction and PCR analysis of genomic DNA from genetically modified pollen in honey. The present report documents on an extraction method for isolation and analysis of pollen DNA present in honey, including the isolation and analysis of isolated genomic pollen DNA using real-time PCR on commercial honey samples and honey samples spiked with various levels of GM MON 810 pollen.
2012-12-20
Publications Office of the European Union
JRC75452
978-92-79-26481-8,   
1831-9424,   
EUR 25524 EN,    OP LB-NA-25524-EN-N,   
http://gmo-crl.jrc.ec.europa.eu/,    https://publications.jrc.ec.europa.eu/repository/handle/JRC75452,   
10.2788/50503,   
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