Report on the Single-Laboratory Validation of a DNA Extraction Method from Carnation Leaves - Validation Report and
Validated Method
In the context of the application for marketing submitted by Florigene Pty Ltd for a genetically modified carnation line (C/NL/09/01) IFD-25958-3, the European Union Reference Laboratory for GM Food and Feed (EU-RL GMFF) has carried out a single-laboratory validation to assess the performance of a DNA extraction protocol to extract genomic DNA from carnation plant leaves for subsequent polymerase chain reaction (PCR) based detection methods and its applicability on the samples provided by the applicant.
This report describes the results of tests carried out by the EU-RL GMFF on samples provided by the method developer and according to the DNA extraction method described by the applicant.
The data reported confirm that the extraction method, applied to samples of carnation leaves, produces DNA of suitable quantity and quality for subsequent PCR-based methods.
JACCHIA Sara;
BOGNI Alessia;
SAVINI Cristian;
MAZZARA Marco;
KREYSA Joachim;
2013-01-11
Publications Office of the European Union
JRC77732
978-92-79-28082-5,
1831-9424,
EUR 25683,
OP LB-NA-25683-EN-N,
https://publications.jrc.ec.europa.eu/repository/handle/JRC77732,
10.2788/76844,
Additional supporting files
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