Title: Singlet oxygen plays a key role in the toxicity and DNA damage caused by nanometric TiO2 in human keratinocytes
Authors: FENOGLIO IvanaPONTI JessicaALLOA ElisaGHIAZZA MaraCORAZZARI IngridCAPOMACCIO ROBIN BRUNOREMBGES DianaOLIARO BOSSO SimonettaROSSI Francois
Citation: NANOSCALE vol. 5 no. 14 p. 6567-6576
Publisher: ROYAL SOC CHEMISTRY
Publication Year: 2013
JRC N°: JRC78852
ISSN: 2040-3364
URI: http://www.ncbi.nlm.nih.gov/pubmed/23760471
http://publications.jrc.ec.europa.eu/repository/handle/JRC78852
DOI: 10.1039/c3nr01191g
Type: Articles in periodicals and books
Abstract: Nanometric TiO2 has been reported to be cytotoxic and genotoxic in different in vitro models when activated by UV light. However, a clear picture of the species mediating the observed toxic effects is still missing. Here, a nanometric TiO2 powder has been modified at the surface to completely inhibit its photo-catalytic activity and to inhibit the generation of all reactive species except for singlet oxygen. The prepared powders have been tested for their ability to induce strand breaks in plasmid DNA and for their cytotoxicity and genotoxicity toward human keratinocyte (HaCaT) cells (100–500 mg mL1, 15 min UVA/B exposure at 216–36 mJ m2 respectively). The data reported herein indicate that the phototoxicity of TiO2 is mainly triggered by particle-derived singlet oxygen. The data presented herein contribute to the knowledge of structure–activity relationships which are needed for the design of safe nanomaterials.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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