Please use this identifier to cite or link to this item:
|Title:||Development of a real-time PCR method for the simultaneous detection of mackerel and horse mackerel|
|Authors:||PRADO Marta; BOIX SANFELIU Ana; VON HOLST Christoph|
|Citation:||FOOD CONTROL vol. 34 no. 1 p. 19-23|
|Publisher:||ELSEVIER SCI LTD|
|Type:||Articles in periodicals and books|
|Abstract:||We have developed a real-time PCR method for the simultaneous detection of horse mackerel (Trachurus trachurus) and mackerel (Scomber scombrus) DNA. This method has been designed to be used as an extension to our previously published method for the simultaneous detection of several fish species. A fragment of the parvalbumin gene was used as target for the development of the reported method. The results showed the required specificity, efficiency of the real-time PCR reaction and comparable selectivity with the previously reported method, therefore their possible complementarity for food and feed analysis is discussed. Additionally, the developed method combined with melt curve analysis allows the differentiation between both fish species which can have interesting applications for authenticity studies.|
|JRC Directorate:||Health, Consumers and Reference Materials|
Files in This Item:
There are no files associated with this item.
Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.