Title: Development and validation of a rapid multiplex ELISA for pyrrolizidine alkaloids and their N-oxides in honey and feed
Authors: OPLATOWSKA-STACHOWIAK MichalinaELLIOTT Christopher T.HUET Anne-CatherineMCCARTHY MarkMULDER Patrick P JVON HOLST ChristophDELAHAUT PhilippeVAN EGMOND HansCAMPBELL Katrina
Citation: ANALYTICAL AND BIOANALYTICAL CHEMISTRY vol. 406 no. 3 p. 757-770
Publisher: SPRINGER HEIDELBERG
Publication Year: 2014
JRC N°: JRC79980
ISSN: 1618-2642
URI: http://link.springer.com/article/10.1007%2Fs00216-013-7488-7
http://publications.jrc.ec.europa.eu/repository/handle/JRC79980
DOI: 10.1007/s00216-013-7488-7
Type: Articles in periodicals and books
Abstract: Pyrrolizidine alkaloids (PAs) are a group of plant secondary metabolites with carcinogenic and hepatotoxic properties. When PA-producing plants contaminate crops, toxins can be transferred through the food chain and cause illness in humans and animals, most notably hepatic venoocclusive disease. Honey has been identified as a direct risk of human exposure. The European Food Safety Authority has recently identified four groups of PAs that are of particular importance for food and feed: senecionine-type, lycopsaminetype, heliotrine-type and monocrotaline-type. Liquid or gas chromatography methods are currently used to detect PAs but there are no rapid screening assays available commercially. Therefore, the aim of this study was to develop a rapid multiplex ELISA test for the representatives of three groups of alkaloids (senecionine, lycopsamine and heliotrine types) that would be used as a risk-management tool for the screening of these toxic compounds in food and feed. The method was validated for honey and feed matrices and was demonstrated to have a detection capability less than 25 μg/kg for jacobine, lycopsamine, heliotrine and senecionine. The zinc reduction step introduced to the extraction procedure allows for the additional detection of the presence of N-oxides of PAs. This first multiplex immunoassay for PA detection with N-oxide reduction can be used for the simultaneous screening of 21 samples for >12 PA analytes. Honey samples (n =146) from various origins were analysed for PA determination. Six samples were determined to contain measurable PAs >25 μg/kg by ELISAwhich correlated to >10 μg/kg by LC-MS/MS.
JRC Directorate:Health, Consumers and Reference Materials

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