Title: Objective scoring of transformed foci in BALB/c 3T3 cell transformation assay by statistical image descriptors
Authors: URANI ChiaraCORVI RaffaellaCALLEGARO G.STEFANINI Federico Mattia
Citation: TOXICOLOGY IN VITRO vol. 27 no. 6 p. 1905-1912
Publication Year: 2013
JRC N°: JRC82624
ISSN: 0887-2333
URI: http://www.sciencedirect.com/science/article/pii/S0887233313001628
DOI: 10.1016/j.tiv.2013.06.011
Type: Articles in periodicals and books
Abstract: In vitro cell transformation assays (CTAs) have been shown to model important stages of in vivo carcinogenesis and have the potential to predict carcinogenicity in humans. Advantages of CTAs are their ability of revealing both genotoxic and non-genotoxic carcinogens while reducing both experimental costs and the number of animals used. The endpoint of the CTA is foci formation, and requires classification under light microscopy based on morphology. Thus current limitations for the wide adoption of the assay partially depend on a fair degree of subjectivity in foci scoring. An objective evaluation may be obtained after separating foci from background monolayer in the digital image, and quantifying values of statistical descriptors which are selected to capture eye-scored morphological features. The aim of this study was to develop statistical descriptors to be applied to transformed foci of BALB/c 3T3, which cover foci size, multilayering and invasive cell growth into the background monolayer. Proposed descriptors were applied to a database of 407 foci images to explore the numerical features, and to illustrate open problems and potential solutions.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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