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dc.contributor.authorSABBIONI E.en_GB
dc.contributor.authorFORTANER TORRENT SALVADORen_GB
dc.contributor.authorFARINA MASSIMOen_GB
dc.contributor.authorDEL TORCHIO RICCARDOen_GB
dc.contributor.authorOLIVATO IOLANDAen_GB
dc.contributor.authorPETRARCA CLAUDIAen_GB
dc.contributor.authorBERNARDINI GIOVANNIen_GB
dc.contributor.authorMARIANI-COSTANTINI RENATOen_GB
dc.contributor.authorPERCONTI SILVIAen_GB
dc.contributor.authorDI GIAMPAOLO LUCAen_GB
dc.contributor.authorGORNATI ROSALBAen_GB
dc.contributor.authorDI GIOACCHINO M.en_GB
dc.identifier.citationNANOTOXICOLOGY vol. 8 no. 4 p. 455-465en_GB
dc.identifier.issn1743-5390 (online)en_GB
dc.description.abstractWe previously described the behaviour of different cobalt forms, i.e., cobalt nanoparticles (CoNP), cobalt microparticles (CoMP) and cobalt ions (Co2+), in culture medium (dissolution, interaction with medium components, bioavailability) as well as their uptake and intracellular distribution in Balb/3T3 mouse fibroblasts (Sabbioni, Nanotoxicology, 2012). Here, we assess the cytotoxicity and morphological transformation of CoNP compared not only to Co2+, but also to CoMP and to released Co products. Cytotoxicity reached maximum at 4-h exposure, with ranking CoMP > CoNP > Co2+. However, if we consider toxicity as a function of intracellular Co, toxicity of the ionic forms seems to prevail over the particles. Co forms other than Co2+ released from particles had toxicity intermediate between particles and ions. Alterations in concentrations of essential elements (Cu, Mg, Zn) in cells exposed to Co particles may contribute to toxicity. Both CoMP and CoNP (but not Co2+ and other released Co forms) induced morphological transformation (CoMP > CoNP). This was dependent on reactive oxygen species production and lipid peroxidation, as indicated by inhibition of type III foci with ascorbic acid. The present results uggest that the previously demonstrated massive mitochondrial and nuclear Co internalisation and DNA adduct formation by CoMP and CoNP (Sabbioni, Nanotoxicology, 2012) induce toxicity and transformation. On the contrary, the role of ions released by particles in culture medium is negligible. Thus, both the chemical and the physical properties of Co particles contribute to cytotoxicity and morphological transformation.en_GB
dc.description.sponsorshipJRC.F.3-Chemicals Safety and Alternative Methodsen_GB
dc.titleCytotoxicity and morphological transforming potential of cobalt nanoparticles, microparticles and ions in Balb/3T3 mouse fibroblasts: an in vitro model.en_GB
dc.typeArticles in periodicals and booksen_GB
dc.identifier.doi10.3109/17435390.2013.796538 (online)en_GB
JRC Directorate:Health, Consumers and Reference Materials

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