DNA double strand break assessment by yH2AX-foci quantification after alpha-particle emitter Ac-225 and electron emitter Lu-177 somatostatin receptor targeted radiotherapy correlates with cell death and apoptosis in vitro
Alpha-particle emitters display high linear energy transfer combined with short range and are promising for treatment of metastasized tumors. Indeed, efficiency and high specificity of tumor-specific antibodies labelled with alpha emitting nuclides were described in vitro and in vivo. Radiolabeled somatostatin analogues with high affinity to somatostatin receptor overexpressing tumors are an attractive option for peptide-receptor radionuclide therapy of metastasized neuroendocrine tumors and great clinical success has been demonstrated with Lutetium-177 as therapeutic isotope.
In this study, we investigated cellular effects of the alpha-particle emitter Actinium-225 in comparison to the beta-particle emitter Lutetium-177 labelled DOTATOC in vitro. Cellular studies were performed in p53 dependent and somatostatin receptor expressing rat pancreatic acinar carcinoma cells (AR42J).
GRAF Franziska;
FAHRER Jörg;
MAUS Stephan;
MORGENSTERN Alfred;
BRUCHERTSEIFER Frank;
VENKATACHALAM S.;
FOTTNER C.;
WEBER M.M.;
HUELSENBECK Johannes;
SCHRECKENBERGER M;
KAINA B;
MIEDERER Matthias;
2013-06-26
Oak Ridge National Laboratory
JRC82791
https://register.ornl.gov/2013/TATS/index.shtml,
https://publications.jrc.ec.europa.eu/repository/handle/JRC82791,
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