Microbicidal Power of Alpha Radiation in Sterilizing Germinating Bacillus anthracis Spores
Radioimmunotherapy (RIT) takes advantage of the specificity and affinity of the antigen21 antibody interaction to deliver microbicidal radioactive nuclides to a site of infection. In this study, we investigated the microbicidal properties of an alpha-emitting 213Bi-labeled mAb, EA2-1, that binds to the immunodominant antigen on Bacillus anthracis spores. Our results showed that dormant spores were highly resistant to 213Bi-EA2-1. Significant spore killing was observed following treatment with 300 μCi labeled 213Bi-EA2-1, however, this effect was not dependent on the mAb. In contrast, when spores were allowed to partially germinate, 213Bi-EA2-1 mediated mAb-specific killing was observed in a dose dependent manner. Dormant spores are very resistant to RIT and germinating spores can be effectively targeted by this treatment.
RIVERA J.;
MORGENSTERN Alfred;
BRUCHERTSEIFER Frank;
KEARNEY John;
TURNBOUGH Charles;
DADACHOVA E.;
CASADEVALL Arturo;
2014-03-26
AMER SOC MICROBIOLOGY
JRC82866
0066-4804,
http://aac.asm.org/content/58/3/1813,
https://publications.jrc.ec.europa.eu/repository/handle/JRC82866,
10.1128/AAC.01266-13,
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