Title: Report on the Verification of the Performance of a Testing Strategy for the Detection of Wheat MON71800 Event Using Real-Time PCR
Authors: SAVINI CristianMAZZARA MarcoLIEVENS ANTOONNARDINI ELENAPETRILLO MAUROANGERS ALEXANDREPATAK DENNSTEDT AlexandreKREYSA JOACHIM
Publisher: Publications Office of the European Union
Publication Year: 2013
JRC N°: JRC83648
ISBN: 978-92-79-32497-0
ISSN: 1831-9424
Other Identifiers: EUR 26070
OPOCE LB-NA-26-070-EN-N
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC83648
DOI: 10.2788/86096
Type: EUR - Scientific and Technical Research Reports
Abstract: In response to a request of DG SANCO to provide National Reference Laboratories (NRLs) as soon as possible with a method to test soft white wheat consignments for the presence of unauthorised GM glyphosate-resistant wheat harbouring the event MON71800, the European Union Reference Laboratory for Genetically Modified Food and Feed (EU-RL GMFF) developed, in collaboration with the European Network of GMO Laboratories (ENGL), a testing strategy intended to be immediately implementable by EU NRLs. The testing strategy is based on a combination of three validated screening methods that allow excluding (detectable) presence of Monsanto’s GM glyphosate-resistant wheat (MON71800) in wheat grain or food/feed products and confirming its presence whenever other GMOs can be excluded. The present report describes the results of the tests carried out by the EU-RL GMFF to verify the testing strategy proposed; the tests were conducted using the positive control sample represented by a crude DNA lysate of MON71800 provided by Monsanto and genomic DNA samples of genetically modified organisms harbouring the CTP2-CP4epsps element for which a validated event-specific method is available. The sensitivity of the three methods was assessed by verifying the relative limit of detection (LODrel) on MON71800 wheat DNA. The LODrel is approximately 0.03% for the P-35S and for T-nos methods and 0.06% for the CTP2-CP4epsps method in 300 nanograms of wheat genomic DNA. Further experimental evidence confirmed that the three methods react against genomic DNA extracted from GM events containing the CTP2-CP4epsps element for which a validated event-specific method is available. The experimental verification hereby reported confirmed the validity of the EU-RL GMFF guidance on testing for GM glyphosate-resistant wheat (MON71800) in wheat grain or in food/feed products containing wheat flour originating or consigned from the US, provided that DNA of acceptable quality can be obtained.
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