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dc.contributor.authorMAZZARA Marcoen_GB
dc.contributor.authorBOGNI Alessiaen_GB
dc.contributor.authorFOTI Nicolettaen_GB
dc.contributor.authorVAN DEN EEDE Guyen_GB
dc.identifier.otherEUR 26152en_GB
dc.identifier.otherOPOCE LB-NA-26152-EN-Nen_GB
dc.description.abstractThe JRC as European Union Reference Laboratory for the GM Food and Feed (EURL-GMFF), established by Regulation (EC) No 1829/2003, in collaboration with the European Network of GMO Laboratories (ENGL), has carried out a collaborative study to assess the performance of a quantitative event-specific method to detect and quantify the MON 531 transformation event in cotton DNA (unique identifier MON-ØØ531-6). The collaborative trial was conducted according to internationally accepted guidelines (1, 2). In accordance with Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and with Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Monsanto provided the detection method and the samples (cotton seeds containing the transformation event and conventional cotton seeds). The JRC prepared the validation samples (calibration samples and blind samples at unknown GM percentage [DNA/DNA]). The collaborative trial involved twelve laboratories from seven European countries. The results of the international collaborative trial mostly met the ENGL performance requirements. The method is therefore considered applicable to the control samples provided, in accordance with the requirements of Annex I-2.C.2 to Commission Regulation (EC) No 641/2004en_GB
dc.description.sponsorshipJRC.I.3-Molecular Biology and Genomicsen_GB
dc.publisherPublications Office of the European Unionen_GB
dc.titleEvent-specific Method for the Quantification of Cotton Line MON 531 Using Real-time PCR - Validation Report and Validated Method – v. 1.01en_GB
dc.typeEUR - Scientific and Technical Research Reportsen_GB
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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