Title: Event-specific Method for the Quantification of Soybean Line MON 89788 Using Real-time PCR v. 1.01: Validation Report and Validated Method
Authors: CHARLES DELOBEL ChrystèleBOGNI AlessiaPINSKI GregorMAZZARA MarcoVAN DEN EEDE Guy
Publisher: Publications Office of the European Union
Publication Year: 2013
JRC N°: JRC84189
ISBN: 978-92-79-33078-0
ISSN: 1831-9424
Other Identifiers: EUR 26153
OPOCE LB-NA-26153-EN-N
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC84189
DOI: 10.2788/20464
Type: EUR - Scientific and Technical Research Reports
Abstract: The JRC as European Union Reference Laboratory for GM Food and Feed (EURL-GMFF), established by Regulation (EC) No 1829/2003, in collaboration with the European Network of GMO Laboratories (ENGL), has carried out a collaborative study to assess the performance of a quantitative event-specific method to detect and quantify the MON89788 transformation event in soybean DNA (unique identifier MON-89788-1). The collaborative trial was conducted according to internationally accepted guidelines (1, 2). In accordance with Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and with Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Monsanto provided the detection method and the samples (soybean seeds containing the transformation event and conventional soybean seeds). The JRC prepared the validation samples (calibration samples and blind samples at unknown GM percentage [DNA/DNA]). The collaborative trial involved twelve laboratories from eight European countries. The results of the international collaborative trial met the ENGL performance requirements. The method is therefore considered applicable to the control samples provided, in accordance with the requirements of Annex I-2.C.2 to Commission Regulation (EC) No 641/2004.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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