Event-specific Method for the Quantification of Soybean Line MON 89788 Using Real-time PCR v. 1.01: Validation Report and Validated Method
The JRC as European Union Reference Laboratory for GM Food and Feed (EURL-GMFF), established by Regulation (EC) No 1829/2003, in collaboration with the European Network of GMO Laboratories (ENGL), has carried out a collaborative study to assess the performance of a quantitative event-specific method to detect and quantify the MON89788 transformation event in soybean DNA (unique identifier MON-89788-1). The collaborative trial was conducted according to internationally accepted guidelines (1, 2).
In accordance with Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and with Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Monsanto provided the detection method and the samples (soybean seeds containing the transformation event and conventional soybean seeds). The JRC prepared the validation samples (calibration samples and blind samples at unknown GM percentage [DNA/DNA]). The collaborative trial involved twelve laboratories from eight European countries.
The results of the international collaborative trial met the ENGL performance requirements. The method is therefore considered applicable to the control samples provided, in accordance with the requirements of Annex I-2.C.2 to Commission Regulation (EC) No 641/2004.
CHARLES DELOBEL Chrystèle;
BOGNI Alessia;
PINSKI Gregor;
MAZZARA Marco;
VAN DEN EEDE Guy;
2013-10-30
Publications Office of the European Union
JRC84189
978-92-79-33078-0,
1831-9424,
EUR 26153,
OP LB-NA-26153-EN-N,
https://publications.jrc.ec.europa.eu/repository/handle/JRC84189,
10.2788/20464,
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