Title: Uncertainties of Size Measurements in Electron Microscopy Characterization of Nanomaterials in Foods
Citation: FOOD CHEMISTRY vol. 176 p. 472–479
Publication Year: 2015
JRC N°: JRC84682
ISSN: 0308-8146
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC84682
DOI: 10.1016/j.foodchem.2014.12.071
Type: Articles in periodicals and books
Abstract: This paper describes the development and validation of electron microscopy procedures for the detection and quantification of nanomaterials in foods. The study addressed a number of key issues relating to the measurement of nanomaterials with broad and narrow particle size distributions in solid and liquid food matrices. A method for predicting uncertainty in particle size measurement related to small sample intake in electron microscope is provided. Other validation parameters, including the limit of detection, recovery, and precision of particle size measurement are determined. The contribution of the subsequent stages in data acquisition process to the measurement of uncertainty is also investigated. The analysis showed that sampling was the main factor contributing to uncertainty in nanomaterial size measurements in food products. This emphasizes the importance of replication over extensive particle counts per replicate in experimental designs for the use of electron microscopy for sizing of nanoparticles in complex matrices such as food. The study is expected to contribute to the future work involving the use of electron microscopy for measurement of nanomaterials in food and other complex matrices.
JRC Directorate:Health, Consumers and Reference Materials

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