Aim: Targeted biocompatible nanoplatforms presenting multiple therapeutic functions have great potential for the treatment of cancer. Materials & methods: Multifunctional nanocomposites formed by polymeric nanoparticles (PNPs) containing two cytotoxic agents – the drug alisertib and silver nanoparticles – were synthesized. These PNPs have been conjugated with a chlorotoxin, an active targeting 36-amino acid-long peptide that specifically binds to MMP‑2, a receptor overexpressed by brain cancer cells. Results: The individual and synergistic activity of these two cytotoxic agents against glioblastoma multiforme was tested both in vitro and in vivo. The induced cytotoxicity in a human glioblastoma–astrocytoma epithelial‑like cell line (U87MG) was studied in vitro through a trypan blue exclusion test after 48 and 72 h of exposure. Subsequently, the PNPs’ biodistribution in healthy animals and their effect on tumor reduction in tumor‑bearing mice were studied using PNPs radiolabeled with 99mTc. Conclusion: Tumor reduction was achieved in vivo when using silver/alisertib@PNPs–chlorotoxin.
LOCATELLI Erica;
NADDAKA Maria;
UBOLDI Chiara;
LOUDOS George;
FRAGOGEORGI Eirini;
MOLINARI Valerio;
PUCCI Andrea;
TSOTAKOS Theodoros;
PSIMADAS Dimitrios;
PONTI Jessica;
COMES FRANCHINI Mauro;
2014-07-09
FUTURE MEDICINE LTD
JRC84754
1743-5889,
http://www.futuremedicine.com/doi/abs/10.2217/nnm.14.1,
https://publications.jrc.ec.europa.eu/repository/handle/JRC84754,
10.2217/NNM.14.1,
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