Title: The preparation and characterization of three solutions of plasmid DNA containing a ruminant-specific fragment with defined copy number concentrations - Reference Materials: IRMM-AD482a,IRMM-AD482b, IRMM-AD482c
Authors: CORBISIER PhilippeMAZOUA StephaneSCARAVELLI ElenaMATRAI JANKAKORTEKAAS Anna MariaCHUNG PUI YAN JENNYTRAPMANN StefanieEMONS Hendrik
Publisher: Publications Office of the European Union
Publication Year: 2013
JRC N°: JRC85135
ISBN: 978-92-79-33521-1
ISSN: 1831-9424
Other Identifiers: EUR 26204
OPOCE LA-NA-26204-EN-N
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC85135
DOI: 10.2787/82135
Type: EUR - Scientific and Technical Research Reports
Abstract: This report describes the processing of a set of plasmid solutions, IRMM-AD482a, b and c. The material was produced following ISO Guide 34:2009. A DNA fragment specific for the identification of ruminant meat were cloned into a pUC18 vector to construct the pIRMM-0103 plasmid. The plasmid was diluted to three different concentration levels. Between unit-homogeneity was quantified and stability during dispatch and storage were assessed in accordance with ISO Guide 35:2006. The materials are intended for the determination of a cut-off value to discriminate positive samples from negative samples by quantitative PCR. As any reference material, the materials can also be used for control charts or validation studies. The RM is available as a set of three plastic tubes containing 1 mL of plasmid solution. The minimum amount of sample to be used is 4 μL.
JRC Directorate:Health, Consumers and Reference Materials

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