Report on the In-house Validation of a DNA Extraction Method from Oilseed rape Grains and Validated Method
In accordance with relevant EU legislation , Pioneer Overseas Corporation provided to the European Union Reference Laboratory for GM Food and Feed (EU-RL GMFF) a DNA extraction method for oilseed rape grains and the relevant samples (ground oilseed rape grains).
In line with its mandate , the EU RL GMFF has conducted an in-house validation of this DNA extraction method. To this end it tested the DNA extraction method on the samples provided and evaluated its performance in terms of DNA yield, integrity and quality.
The in-house validation study confirmed that the method meets the method performance requirements as established by the EU-RL GMFF and the ENGL , and that it satisfies the provisions of Annex I-2.C.2 to Regulation (EC) No 641/2004.
The method is therefore fit for the purpose of producing rapeseed DNA of suitable quantity and quality for subsequent PCR-based analysis.
This report is published at http://gmo-crl.jrc.ec.europa.eu/StatusOfDossiers.htm.
JACCHIA Sara;
BOGNI Alessia;
MAZZARA Marco;
KREYSA Joachim;
2014-01-07
European Commission
JRC85313
978-92-79-34885-3,
1831-9424,
EUR 26382,
OP LB-NA-26382-EN-N,
https://publications.jrc.ec.europa.eu/repository/handle/JRC85313,
10.2788/48066,
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