Report on the Single-laboratory Validation of a PCR-based Detection Method for Identification of Florigene™ 26407 GM Carnation
Suntory Holdings Ltd has submitted an application for marketing (C/NL/09/02) of a genetically
modified carnation line 26407 (Unique identifier: IFD-26407-2). In this context, the European Union
Reference Laboratory for GM Food and Feed (EU-RL GMFF) was asked to carry out a singlelaboratory
validation of the performance of a polymerase chain reaction (PCR)-based method for
detecting and identifying the carnation GM line 26407, developed by the applicant.
This report describes the results of this validation, carried out by the EU-RL GMFF with control
samples provided by the applicant.
The method is a duplex end-point PCR, where a carnation (taxon) target and a transgenic
sequence are detected simultaneously.
The limit of detection (LOD) of the method has been established to be at least 10 copies for the
taxon-specific target and between 50 and 10 copies for the GM target, based on haploid genome
copy number. The event-specificity of the method was assessed by the applicant as being
sufficient.
The EU-RL could verify that the taxon-specific primers correctly detect the endogenous gene target
in genomic DNA of a conventional carnation line (negative control) and in the genomic DNA of the
GM carnation line, while the GM target is detected by the GM specific primers only in genomic DNA
of 26407 GM line (positive control).
SAVINI Cristian;
PINSKI Gregor;
MAZZARA Marco;
KREYSA Joachim;
2013-11-29
Publications Office of the European Union
JRC85668
978-92-79-34531-9,
1831-9424,
EUR 26290,
OP LB-NA-26290-EN-N,
https://publications.jrc.ec.europa.eu/repository/handle/JRC85668,
10.2788/34771,
Additional supporting files
| File name | Description | File type | |
|---|
