Interactions of Serum Derived Proteins with Sub-Micrometer Structured Surfaces

Protein surface-interactions are of particular importance in the field of nanosensors and nanotoxicology. The ability to position and keep orientation and activity of proteins on nanostructures is essential for the proper function of nanosensors. It is also well known that proteins adsorbed on surfaces of nanoparticles will mediate their adsorption and uptake by the cells. Furthermore, conformational changes of the proteins, and thus the modification of their biological functions, will depend on the radius of curvature of the surface: this aspect is therefore of particular importance on nanomaterials or nanostructures. In both cases, methods for studying and controlling the adsorption of proteins on surfaces at the nanoscale are needed. This report presents the development and optimization of two nanofabrication methods combining plasma polymerization and electron beam lithography techniques for producing chemical patterns at nanoscale. Patterned surfaces produced with both fabrication processes exhibit high binding capacity as tested by Surface Plasmon Resonance with model proteins. Furthermore, we show that adsorption on the nanopatterns is similar to that observed on a flat surface with a direct proportionality to the active area. This work shows the suitability of the chemical patterning techniques and their high potential applicability for protein surface interactions study and miniaturized biosensing device development.

PEREZ ROLDAN Maria Jesus;  PARRACINO Antonietta;  CECCONE Giacomo;  COLPO Pascal;  ROSSI Francois; 

2014-06-19

WILEY-V C H VERLAG GMBH

JRC86404

1612-8850,   

http://onlinelibrary.wiley.com/doi/10.1002/ppap.201300174/abstract,    jsessionid=6AD2F52AE349D53B7A442315A635E52D.f03t02,    https://publications.jrc.ec.europa.eu/repository/handle/JRC86404,   

10.1002/ppap.201300174,