Please use this identifier to cite or link to this item:
|Title:||Developmental stage dependent neural stem cells sensitivity to methylmercury chloride on different biofunctional surfaces|
|Authors:||ZYCHOWICZ M.; DZIEDZICKA D.; MEHN Dora; KOZLOWSKA H.; KINSNER-OVASKAINEN Agnieszka; STEPIEN P.p.; ROSSI Francois; BUZANSKA Leonora|
|Citation:||TOXICOLOGY IN VITRO vol. 28 no. 1 p. 76-87|
|Publisher:||PERGAMON-ELSEVIER SCIENCE LTD|
|Type:||Articles in periodicals and books|
|Abstract:||Sensitivity of neural stem cells viability, proliferation and differentiation upon exposure to methylmercury chloride (MeHgCl) was investigated on different types of biofunctional surfaces. Patterns of biodomains created by microprinting/microspotting of poly-L-lysine or extracellular matrix proteins (fibronectin and vitronectin) allowed for non-specific electrostatic or specific, receptor mediated interactions, respectively, between stem cells and the surface. The neural stem cell line HUCB-NSC has been previously shown to be susceptible to MeHgCl in developmentally dependent manner. Here we demonstrated that developmental sensitivity of HUCB-NSC to MeHgCl depends upon the type of adhesive biomolecules and the geometry of biodomains. Proliferation of HUCB-NSC was diminished in time and MeHgCl concentration dependent manner. In addition, the response to MeHgCl was found to be cell-type dependent. Undifferentiated cells were the most sensitive independently of the type of bioactive domain. Significant decrease of GFAP+ cells was detected among cells growing on poly-L-lysine, while on fibronectin and vitronectin, this effect was observed only in the highest (1 lM) concentration of MeHgCl. b-Tubulin III expressing cells were most sensitive on fibronectin domains. In addition, limited bioactive domains to lm in size, as compared to non-patterned larger area of the same adhesive substrate, exerted protective role. Thus, the surface area and type of cell/biofunctional surface interaction exerted significant influence on developmental stage and cell-type specific response of HUCB-NSC to MeHgCl.|
|JRC Directorate:||Institute for Health and Consumer Protection Historical Collection|
Files in This Item:
There are no files associated with this item.
Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.