Sensitivity of neural stem cells viability, proliferation and differentiation upon exposure to methylmercury chloride (MeHgCl) was investigated on different types of biofunctional surfaces. Patterns of biodomains created by microprinting/microspotting of poly-L-lysine or extracellular matrix proteins (fibronectin and vitronectin) allowed for non-specific electrostatic or specific, receptor mediated interactions, respectively, between stem cells and the surface. The neural stem cell line HUCB-NSC has been previously shown to be susceptible to MeHgCl in developmentally dependent manner. Here we demonstrated that developmental sensitivity of HUCB-NSC to MeHgCl depends upon the type of adhesive biomolecules and the geometry of biodomains. Proliferation of HUCB-NSC was diminished in time and MeHgCl concentration dependent manner. In addition, the response to MeHgCl was found to be cell-type dependent. Undifferentiated cells were the most sensitive independently of the type of bioactive domain. Significant decrease of GFAP+ cells was detected among cells growing on poly-L-lysine, while on fibronectin and vitronectin, this effect was observed only in the highest (1 lM) concentration of MeHgCl. b-Tubulin III expressing cells were most sensitive on fibronectin domains. In addition, limited bioactive domains to lm in size, as compared to non-patterned larger area of the same adhesive substrate, exerted protective role. Thus, the surface area and type of cell/biofunctional surface interaction exerted significant influence on developmental stage and cell-type specific response of HUCB-NSC to MeHgCl.

ZYCHOWICZ M.;  DZIEDZICKA D.;  MEHN Dora;  KOZLOWSKA H.;  KINSNER-OVASKAINEN Agnieszka;  STEPIEN P.P.;  ROSSI Francois;  BUZANSKA Leonora; 

2013-12-19

PERGAMON-ELSEVIER SCIENCE LTD

JRC86572

0887-2333,   

https://publications.jrc.ec.europa.eu/repository/handle/JRC86572,   

10.1016/j.tiv.2013.06.023,