Title: Fast cell counting – the better cell counting?
Authors: LIPŞA DORELIACACHO CarmenREMBGES DianaBARRERO Josefa
Citation: FRESENIUS ENVIRONMENTAL BULLETIN vol. 23 no. 12 p. 3054-3058
Publisher: PARLAR SCIENTIFIC PUBLICATIONS (P S P)
Publication Year: 2014
JRC N°: JRC87008
ISSN: 1018-4619
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC87008
Type: Articles in periodicals and books
Abstract: A better understanding of environmental factors influencing human health will benefit from the availability of fast and reliable methods allowing the quasi simultaneous measurement of a panel of endpoints in cell exposure studies. Automated cell-counting methods are basic for this purpose if comparability with traditional manual cell counting procedure is documented. In the present paper, both dead and viable cells of human lung adenocarcinoma epithelial cell line (A549) and human bronchial epithelial cell line (16HBE 14o-) at various cell densities ranging from 50 000 to 400 000 cells/ml, are counted by both Trypan blue dye exclusion staining counted with a hemocytometer and a handheld automated cell counter based on the Coulter principle (Scepter 2.0, Millipore). Correlation indexes higher than 0.998 have been obtained using both cell counting protocols in all cases, thus confirming the reliable use of automated cell counters whenever fast, easy to use, precise cell counting is a requisite.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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