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dc.contributor.authorLIPŞA DORELIAen_GB
dc.contributor.authorCACHO Carmenen_GB
dc.contributor.authorREMBGES Dianaen_GB
dc.contributor.authorBARRERO Josefaen_GB
dc.date.accessioned2015-03-31T00:03:11Z-
dc.date.available2015-03-30en_GB
dc.date.available2015-03-31T00:03:11Z-
dc.date.created2015-03-26en_GB
dc.date.issued2014en_GB
dc.date.submitted2013-12-11en_GB
dc.identifier.citationFRESENIUS ENVIRONMENTAL BULLETIN vol. 23 no. 12 p. 3054-3058en_GB
dc.identifier.issn1018-4619en_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC87008-
dc.description.abstractA better understanding of environmental factors influencing human health will benefit from the availability of fast and reliable methods allowing the quasi simultaneous measurement of a panel of endpoints in cell exposure studies. Automated cell-counting methods are basic for this purpose if comparability with traditional manual cell counting procedure is documented. In the present paper, both dead and viable cells of human lung adenocarcinoma epithelial cell line (A549) and human bronchial epithelial cell line (16HBE 14o-) at various cell densities ranging from 50 000 to 400 000 cells/ml, are counted by both Trypan blue dye exclusion staining counted with a hemocytometer and a handheld automated cell counter based on the Coulter principle (Scepter 2.0, Millipore). Correlation indexes higher than 0.998 have been obtained using both cell counting protocols in all cases, thus confirming the reliable use of automated cell counters whenever fast, easy to use, precise cell counting is a requisite.en_GB
dc.description.sponsorshipJRC.I.1-Chemical Assessment and Testingen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherPARLAR SCIENTIFIC PUBLICATIONS (P S P)en_GB
dc.relation.ispartofseriesJRC87008en_GB
dc.titleFast cell counting – the better cell counting?en_GB
dc.typeArticles in periodicals and booksen_GB
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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