Report on the development of a method for the determination of Alternaria toxins and citrinin in wheat, tomato juice and sunflower seeds by liquid chromatography – tandem mass spectrometry
A new method was developed for the determination of five Alternaria toxins and citrinin in wheat, tomato juice and sunflower seed samples based on a liquid chromatography – tandem mass spectrometric determination. The optimized sample preparation involved a solid – liquid extraction with pure methanol, a derivatization step, and subsequent, solid-phase extraction purification on Strata-XL cartridges. The separation was carried out using HPLC column packed with core-shell C-18 particles. HPLC system was directly coupled to mass spectrometer via electrospray interface employed in negative mode. For the highest selectivity and sensitivity multiply reaction monitoring mode was used in the detector. The absolute recoveries (63.3% – 113.1%) and repeatability (1.2% – 13.9%) of the method, calculated from spiked samples, generated sufficient data.
TOELGYESI Adam;
STROKA Joerg;
2014-01-22
Publications Office of the European Union
JRC88087
978-92-79-35423-6,
1831-9424,
EUR 26496,
OP LA-NA-26496-EN-N,
https://publications.jrc.ec.europa.eu/repository/handle/JRC88087,
10.2787/88794,
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