Title: Different mechanisms are involved in oxidative DNA damage and genotoxicity induction by ZnO and TiO2 nanoparticles in human colon carcinoma cells
Authors: ZIJNO AndreaDE ANGELIS IsabellaDE BERARDIS BarbaraANDREOLI CristinaRUSSO Maria TeresaPIETRAFORTE DonatellaSCORZA GiuseppeDEGAN PaoloPONTI JessicaROSSI FrancoisBARONE Flavia
Citation: TOXICOLOGY IN VITRO vol. 29 p. 1503-1512
Publication Year: 2015
JRC N°: JRC88474
ISSN: 0887-2333
URI: http://www.sciencedirect.com/science/article/pii/S0887233315001459
DOI: 10.1016/j.tiv.2015.06.009
Type: Articles in periodicals and books
Abstract: In this work we investigated the genotoxicity of zinc oxide and titanium dioxide nanoparticles (ZnO NPs; TiO2 NPs) induced by oxidative stress on human colon carcinoma cells (Caco-2 cells). We measured free radical production in acellular conditions by Electron Paramagnetic Resonance technique and genotoxicity by micronucleus and Comet assays. Oxidative DNA damage was assessed by modified Comet assay and by measuring 8-oxodG steady state levels. The repair kinetics of DNA oxidation as well as the expression levels of hOGG1 were also analyzed. Even if both NPs were able to produce ROS in acellular conditions and to increase 8-oxodG levels in Caco-2 cells, only ZnO NPs resulted genotoxic inducing micronuclei and DNA damage. Furthermore, Caco-2 cells exposed to ZnO NPs were not able to repair the oxidative DNA damage that was efficiently repaired after TiO2 NPs treatment, through OGG1 involvement. These results indicate that the high oxidant environment caused by ZnO NPs in our cellular model can induce DNA damage and affect the repair pathways.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

Files in This Item:
There are no files associated with this item.

Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.