Title: Report on the In-house Validation of a DNA Extraction Method from Soybean Grains and Validated Method
Authors: SAVINI CristianMARETTI MatteoMAZZARA MarcoKREYSA JOACHIM
Publisher: Publications Office of the European Union
Publication Year: 2014
JRC N°: JRC89869
ISBN: 978-92-79-38431-8
ISSN: 1831-9424
Other Identifiers: EUR 26664
OP LB-NA-26664-EN-N
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC89869
DOI: 10.2788/8352
Type: EUR - Scientific and Technical Research Reports
Abstract: In accordance with relevant EU legislation , Dow AgroSciences LLC provided to the European Union Reference Laboratory for GM Food and Feed (EU-RL GMFF) a DNA extraction method for soybean grains and the relevant samples (ground soybean grains). In line with its mandate , the EU RL GMFF has conducted an in-house validation of this DNA extraction method. To this end it tested the DNA extraction method on the samples provided and evaluated its performance in terms of DNA yield, integrity and quality. The in-house validation study confirmed that the method meets the method performance requirements as established by the EU-RL GMFF and the ENGL , and that it satisfies the provisions of Annex I-2.C.2 to Regulation (EC) No 641/2004. The method is therefore fit for the purpose of producing soybean DNA of suitable quantity and quality for subsequent PCR-based analysis.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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