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|Title:||OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing|
|Authors:||BUSQUET Francois; STRECKER Ruben; RAWLINGS Jane; BELANGER Scott; BRAUNBECK Thomas; CARR Greg; CENIJN Peter; FOCHTMAN Przemyslaw; GOURMELON Anne; HÜBLER Nicole; KLEENSANG Andre; KNÖBEL Melanie; KUSSATZ Carola; LEGLER Juliette; LILLICRAP Adam; MARTINEZ-JERONIMO Fernando; POLLEICHTNER Christian; RZODECZKO Helena; SALINAS Edward; SCHNEIDER Katharina E.; SCHOLZ Stefan; VAN DEN BRANDHOF Evert-Jan; VAN DER VEN Leo; WALTER-ROHDE Susanne; WEIGT Stefan; WITTERS Hilda; HALDER MARIA ELISABETH|
|Citation:||REGULATORY TOXICOLOGY AND PHARMACOLOGY vol. 69 p. 496-511|
|Publisher:||ACADEMIC PRESS INC ELSEVIER SCIENCE|
|Type:||Articles in periodicals and books|
|Abstract:||The OECD validation study of the zebrafish embryo acute toxicity test (ZFET) for acute aquatic toxicity testing evaluated the ZFET reproducibility by testing 20 chemicals at 5 different concentrations in 3 independent runs in at least 3 laboratories. Stock solutions and test concentrations were analytically confirmed for 11 chemicals. Newly fertilised zebrafish eggs (20/concentration and control) were exposed for 96 h to chemicals. Four apical endpoints were recorded daily as indicators of acute lethality: coagulation of the embryo, lack of somite formation, non-detachment of the tail bud from the yolk sac and lack of heart-beat. Results (LC50 values for 48/96 h exposure) show that the ZFET is a robust method with a good intra- and inter-laboratory reproducibility (CV < 30 %) for most chemicals and laboratories. The reproducibility was lower (CV > 30 %) for some very toxic or volatile chemicals, and chemicals tested close to their limit of solubility. The ZFET is now available as OECD Test Guideline 236. Considering the high predictive capacity of the ZFET demonstrated by Belanger et al (2013, ETC 32:1768-1783) in their retrospective analysis of acute fish toxicity and fish embryo acute toxicity data, the ZFET is ready to be considered for acute fish toxicity for regulatory purposes.|
|JRC Directorate:||Institute for Health and Consumer Protection Historical Collection|
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