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dc.contributor.authorSACCO Maria-Graziaen_GB
dc.contributor.authorGATTO FRANCESCOen_GB
dc.contributor.authorPARACCHINI VALENTINAen_GB
dc.contributor.authorSCARAVELLI Elenaen_GB
dc.contributor.authorNARDINI ELENAen_GB
dc.contributor.authorSAVINI Cristianen_GB
dc.contributor.authorMAZZARA Marcoen_GB
dc.contributor.authorKREYSA JOACHIMen_GB
dc.date.accessioned2014-10-04T00:02:34Z-
dc.date.available2014-06-19en_GB
dc.date.available2014-10-04T00:02:34Z-
dc.date.created2014-06-17en_GB
dc.date.issued2014en_GB
dc.date.submitted2014-06-06en_GB
dc.identifier.isbn978-92-79-38429-5en_GB
dc.identifier.issn1831-9424en_GB
dc.identifier.otherEUR 26662en_GB
dc.identifier.otherOP LB-NA-26662-EN-Nen_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC90496-
dc.description.abstractCholine Chloride 60% (CC) is a feed additive that is imported in significant quantities from China. In a number of cases GM rice, harbouring the event Bt63, has been found in imported CC batches but not in derived pre-mixes. The Member States were asked to provide positive CC samples and derived pre-mixes to the EU-RL GMFF in order to allow the EU-RL GMFF establishing practical approaches to DNA extraction and the subsequent testing for presence of (a) rice and (b) the Bt63 rice event. This technical note has been derived based on data provided from National Control Laboratories and experience made by the EU-RL GMFF when re-testing nine feed additive samples and ten pre-mixes samples. The EU-RL GMFF found that the extraction of DNA from the feed additive Choline Chloride (CC) does not normally pose specific problems. It can be carried out following the available protocols or using available standard DNA extraction kits. The extraction of DNA from derived pre-mixes (PMCC) is, however, posing problems because of observed strong inhibition and the presence of DNA from additional sources than those present in the CC. The EU-RL GMFF has tried a number of protocols and extraction kits on PMCC samples. On this basis it is concluded that it is advisable to carefully purify the DNA, to verify the possible presence of inhibition effects and eventually to try to reduce any inhibition observed. Concerning testing the extracted DNA for rice and Bt63 rice event presence, this does not pose a problem in case of the CC while for PMCC rice was not anymore detectable or was detected in trace amounts. In any case testing for Bt63 presence was impossible. A possible explanation could be that much of the extracted DNA is from other plants than rice and hence the concentration of rice DNA is below the LOD of the rice-taxon specific method. In order to verify this, the EU-RL GMFF has analysed the available rice (taxon-) specific methods by means of bio-informatics and (partly) in the laboratory and concludes that they all should be suitable for specifically and reliably detecting rice from the Oryza sativa species. The re-testing by the EU-RL GMFF of CC and PMCC samples has confirmed the results of the initial tests.en_GB
dc.description.sponsorshipJRC.I.3-Molecular Biology and Genomicsen_GB
dc.format.mediumOnlineen_GB
dc.languageENGen_GB
dc.publisherPublications Office of the European Unionen_GB
dc.relation.ispartofseriesJRC90496en_GB
dc.titleExtraction of DNA from Choline Chloride Feed Additive (CC) and from derived Pre-Mixes (PMCC) and Screening of CC and PMCC for (a) presence of rice and (b) presence of Bt63en_GB
dc.typeEUR - Scientific and Technical Research Reportsen_GB
dc.identifier.doi10.2788/83319en_GB
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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