Title: Report on the Verification of the Performance of MS8, RF3 and GT73 Event-specific PCR-based Methods Applied to DNA Extracted from GM Stack MS8xRF3xGT73 Oilseed Rape
Authors: NARDINI ELENAMAZZARA MarcoKREYSA JOACHIM
Publisher: Publications Office of the European Union
Publication Year: 2014
JRC N°: JRC91061
ISBN: 978-92-79-44019-9
ISSN: 1831-9424
Other Identifiers: EUR 26926
OP LB-NA-26926-EN-N
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC91061
DOI: 10.2788/356575
Type: EUR - Scientific and Technical Research Reports
Abstract: A joint application was submitted by Bayer CropScience AG and Monsanto Company to request the authorisation of genetically modified stack (GM stack) MS8xRF3xGT73 oilseed rape (tolerant to glufosinate ammonium and glyphosate) and all sub-combinations of the individual events as present in the segregating progeny, for food and feed uses, and import and processing, in accordance with articles 5 and 17 of Regulation (EC) NoN° 1829/2003 on GM Food and GM Feed. The unique identifier assigned to GM stack MS8xRF3xGT73 oilseed rape is ACS-BNØØ5-8xACS-BNØØ3-6xMON-ØØØ73-7. The GM stack MS8xRF3xGT73 oilseed rape has been obtained by conventional crossing between three genetically modified oilseed rape events: MS8, RF3 and GT73, without any new genetic modification. The EU-RL GMFF has previously validated individually, and declared fit for purpose, the detection methods for the single events MS8, RF3 and GT73 (see http://gmo-crl.jrc.ec.europa.eu/StatusOfDossiers.aspx). In line with the approach defined by the ENGL (http://gmo-crl.jrc.ec.europa.eu/doc/Min_Perf_Requirements_Analytical_methods.pdf) the EU-RL GMFF has carried out only an in-house verification of the performance of each validated method when applied to genomic DNA extracted from GM stack MS8xRF3xGT73 oilseed rape. The results of the in-house verification led to the conclusion that the individual methods meet the ENGL performance criteria also when applied to genomic DNA extracted from the GM stack MS8xRF3xGT73 oilseed rape.
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