Differentiation-Promoting Medium Additives for Hepatocyte Cultivation and Cryopreservation

The Good In Vitro Method Principles (GIVIMP) guidance will help to ensure that in vitro methods destined for regulatory use will prove to be robust, reliable and ultimately useful for supporting regulatory decisions. The book chapter on "Differentiation-Promoting Medium Additives for Hepatocyte Cultivation and Cryopreservation, Springer NY Book - Methods in Molecular Biology" gives a contribution to topic 4 of the envisaged GIVIMP guidance that will focus on the key importance of applying Good Cell Culture Practice, essential in the authentication and characterisation of the in vitro biological model (e.g. test systems such as cell lines, primary cells and tissues) used in in vitro methods. This book chapter contributes to the sufficient understanding of primary human hepatocytes and of the relevant factors which could affect differentiation. Isolated primary hepatocytes are considered as the reference system for in vitro hepatic methods. Following the isolation of primary hepatocytes from liver tissue, an unfavorable process named dedifferentiation is initiated leading to the attenuation of the hepatocellular phenotype both at the morphological and functional level. Freshly isolated hepatocytes can be used immediately or can be cryopreserved for future purposes. Currently, a number of antidedifferentiation strategies exist to extend the life span of isolated hepatocytes. The addition of differentiation promoting compounds to the hepatocyte culture medium is the oldest and simplest antidedifferentiation approach applied. In the present chapter, the most commonly used medium additives for cultivation and cryopreservation of primary hepatocytes are reviewed.

GOULIARMOU Varvara;  PELKONEN Olavi;  COECKE Sandra; 

2015-01-09

Springer Science

JRC92230

978-1-4939-2073-0,   

1063-3745,   

https://publications.jrc.ec.europa.eu/repository/handle/JRC92230,