Title: Qualitative and Quantitative Detection of Botulinum Neurotoxins from Complex Matrices: Results of the First International Proficiency Test
Authors: WORBS SylviaFIEBIG UZELENY ReinhardSCHIMMEL HeinzRUMMEL AndreasLUGINBÜHL WernerDORNER Brigitte
Citation: TOXINS vol. 7 no. 12 p. 4935–4966
Publisher: MDPI AG
Publication Year: 2015
JRC N°: JRC92785
ISSN: 2072-6651
URI: http://www.mdpi.com/2072-6651/7/12/4857
http://publications.jrc.ec.europa.eu/repository/handle/JRC92785
DOI: 10.3390/toxins7124857
Type: Articles in periodicals and books
Abstract: In the framework of the EU project EQuATox a first international proficiency test (PT) on the detection and quantification of BoNT was conducted. Sample materials included BoNT serotypes A, B and E spiked into buffer, milk, meat extract and serum. A variety of methods was applied by the participants combining different principles of detection, identification and quantification. Based on qualitative assays, 95% of all results reported were correct. Successful strategies for BoNT detection were based on a combination of complementary immunological, MS-based and functional methods or on suitable functional in vivo / in vitro approaches (mouse bioassay, hemidiaphrama assay, Endopep-MS assay). Quantification of BoNT/A, BoNT/B and BoNT/E was performed by 48% of participating laboratories. It turned out that precise quantification of BoNT was difficult resulting in a substantial scatter of quantitative data. This was especially true for results obtained by the mouse bioassay which is currently seen as “gold standard” for BoNT detection. The results clearly demonstrate the urgent need of certified BoNT reference materials and the development of methods replacing animal testing. In this context, the BoNT PT provided the valuable information that both the Endopep-MS assay and the hemidiaphrama assay delivered quantitative results superior to the mouse bioassay.
JRC Directorate:Health, Consumers and Reference Materials

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