Title: Guidelines for validation of qualitative real-time PCR methods
Authors: BROEDERS SylviaHUBER IngridGROHMANN LutzBERBEN GilbertTAVERNIERS IsabelMAZZARA MarcoROOSENS NancyMORISSET Dany
Citation: TRENDS IN FOOD SCIENCE \& TECHNOLOGY vol. 37 no. 2 p. 115-126
Publisher: ELSEVIER SCIENCE LONDON
Publication Year: 2014
JRC N°: JRC93345
ISSN: 0924-2244
URI: http://www.sciencedirect.com/science/article/pii/S0924224414000661
http://publications.jrc.ec.europa.eu/repository/handle/JRC93345
DOI: 10.1016/j.tifs.2014.03.008
Type: Articles in periodicals and books
Abstract: As for many areas of molecular testing, detection of Genetically Modified Organisms (GMO) relies on the real-time Polymerase Chain Reaction (qPCR) technology. Due to the increasing number of GMO, a screening approach using qualitative screening methods has become an integrated part of GMO detection. However, specific guidelines for the validation of these methods are lacking. Here, a pragmatic approach to conduct in-house and inter-laboratory validation studies for GMO screening methods, is proposed. Such guidelines could be adapted to other areas where qualitative qPCR methods are used for molecular testing allowing to implement easily a more reliable screening phase where necessary.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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