We propose a simple method to determine the structure and morphology of nanoparticle protein complexes. By combining a separation method with online size measurements: density measurements and circular dichroism, we could identify the number of proteins bound to each nano particle and their secondary structure changes in the complex. This method provides much-needed experimental information on the interaction of proteins with nanoparticles next generation targeted nanodrug delivery systems.
CAPOMACCIO Robin Bruno;
OJEA JIMENEZ Isaac;
COLPO Pascal;
GILLILAND Douglas;
CECCONE Giacomo;
ROSSI Francois;
CALZOLAI Luigi;
2016-02-17
ROYAL SOC CHEMISTRY
JRC95144
2040-3364,
http://pubs.rsc.org/en/Content/ArticleLanding/2015/NR/C5NR05147A#!divAbstract,
https://publications.jrc.ec.europa.eu/repository/handle/JRC95144,
10.1039/c5nr05147a,
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