Please use this identifier to cite or link to this item:
|Title:||Highly flexible platform for tuning surface properties of silica nanoparticles and monitoring their biological interaction|
|Authors:||OJEA JIMENEZ ISAAC; URBAN LOPEZ PATRICIA; BARAHONA RUIZ FRANCISCO; PEDRONI MATTEO; CAPOMACCIO ROBIN BRUNO; CECCONE Giacomo; KINSNER-OVASKAINEN Agnieszka; ROSSI Francois; GILLILAND Douglas|
|Citation:||ACS APPLIED MATERIALS & INTERFACES vol. 8 p. 4838−4850|
|Publisher:||AMER CHEMICAL SOC|
|Type:||Articles in periodicals and books|
|Abstract:||The present work offers a simple, reliable and scalable seeding-growth methodology to prepare silica nanoparticles (SiO2 NPs) (20, 30, 50 and 80 nm) directly in aqueous phase, both as plain- as well as fluorescent-labelled silica. The amount of fluorescent label per particle remained constant regardless of size, which facilitates measurements in terms of number-based concentrations. Dispersions of SiO2 NPs were functionalized with an epoxysilane, thus providing a flexible platform for the covalent immobilization of wide variety of molecules under mild experimental conditions. This approach was validated with ethylenediamine and L-Serine to generate positive and negatively charged particles, respectively. Accurate characterization of particle size, size distributions, morphology and surface chemistry is provided, both for as-synthesized particles and after incubation in cell culture medium. The impact of physico-chemical properties of SiO2 NPs was investigated with human alveolar basal epithelial cells (A549) such as the effect in cytotoxicity, cell internalization and membrane interaction.|
|JRC Directorate:||Health, Consumers and Reference Materials|
Files in This Item:
There are no files associated with this item.
Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.