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dc.contributor.authorLA SPINA RITAen_GB
dc.contributor.authorFERRERO VALENTINAen_GB
dc.contributor.authorAIELLO VENERAen_GB
dc.contributor.authorPEDOTTI MATTIAen_GB
dc.contributor.authorVARANI LUCAen_GB
dc.contributor.authorLETTIERI TERESAen_GB
dc.contributor.authorCALZOLAI LUIGIen_GB
dc.contributor.authorHAASNOOT WILLEMen_GB
dc.contributor.authorCOLPO PASCALen_GB
dc.date.accessioned2018-01-14T01:21:11Z-
dc.date.available2018-01-12en_GB
dc.date.available2018-01-14T01:21:11Z-
dc.date.created2018-01-10en_GB
dc.date.issued2018en_GB
dc.date.submitted2015-05-12en_GB
dc.identifier.citationBIOSENSORS vol. 8 no. 1 p. 1en_GB
dc.identifier.issn2079-6374en_GB
dc.identifier.urihttp://www.mdpi.com/2079-6374/8/1/1en_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC95981-
dc.description.abstractChemicals such as Endocrine Disrupting Compounds (EDCs) have shown to interfere with endogenous hormone systems affecting the endocrine, immune and nervous systems of animals, causing many problems including reproductive disorders and cancers. This highlights the urgency for developing fast and sensitive measurements to detect and monitor EDCs, which are detrimental even at very low concentration. This task is challenging since their low molecular weight (typically < 500 Da), complicates the use of classical label free detection techniques. In this work, we show that using rationally designed Estrogen Receptor alpha (as bio-recognition element) together with conformation-sensitive peptides (as amplification agent) allowed detecting 17β-estradiol at 20 ng/L, similar to the concentration value found in real water samples. Furthermore, the combination of wild type ER and selected ER mutants are used to differentially detect and discriminate the presence of agonists (such as 17β-estradiol) and antagonist compounds (such as Tamoxifen) of the ERα in a single experiment. Since 17β-estradiol has been placed in the watch list of substances to be monitored in European Union waters, these results can have a direct impact on the implementation of legislation.en_GB
dc.description.sponsorshipJRC.F.2-Consumer Products Safetyen_GB
dc.format.mediumOnlineen_GB
dc.languageENGen_GB
dc.publisherMULTIDISCIPLINARY DIGITAL PUBLISHING INSTITUTE (MDPI)en_GB
dc.relation.ispartofseriesJRC95981en_GB
dc.titleLabel-Free Biosensor Detection of Endocrine Disrupting Compounds Using Engineered Estrogen Receptorsen_GB
dc.typeArticles in periodicals and booksen_GB
dc.identifier.doi10.3390/bios8010001en_GB
JRC Directorate:Health, Consumers and Reference Materials

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