Development and applicability of a ready-to-use PCR system for GMO screening

With the growing number of GMOs introduced to the market, testing laboratories have seen their workload increase significantly. Ready-to-use multi-target PCR-based detection systems, such as pre-spotted plates (PSP), reduce analysis time while increasing capacity. This paper describes the development and applicability to GMO testing of a screening strategy involving a PSP and its associated web-based Decision Support System. The screening PSP was developed to detect all GMOs authorized in the EU in one single PCR experiment, through the combination of 16 validated assays. The screening strategy was successfully challenged in a wide inter-laboratory study on real-life food/feed samples. The positive outcome of this study could result in the adoption of a PSP screening strategy across the EU; a step that would increase harmonization and quality of GMO testing in the EU. Furthermore, this system could represent a model for other official control areas where high-throughput DNA-based detection systems are needed.

ROSA Sabrina;  GATTO Francesco;  ANGERS Alexandre;  PETRILLO Mauro;  KREYSA Joachim;  QUERCI Maddalena; 

2016-01-26

ELSEVIER SCI LTD

JRC96265

0308-8146,   

http://www.sciencedirect.com/science/article/pii/S0308814616300073,    https://publications.jrc.ec.europa.eu/repository/handle/JRC96265,   

10.1016/j.foodchem.2016.01.007,