Title: Surface analysis of gold nanoparticles functionalized with thiol-modified glucose SAMs for biosensor applications
Authors: SPAMPINATO VALENTINAPARRACINO ANTONIETTALA SPINA RITAROSSI FRANCOISCECCONE GIACOMO
Citation: FRONTIERS IN CHEMISTRY vol. 4 p. 00008
Publisher: FRONTIERS MEDIA SA
Publication Year: 2016
JRC N°: JRC97142
ISSN: 2296-2646
URI: http://journal.frontiersin.org/article/10.3389/fchem.2016.00008/full
http://publications.jrc.ec.europa.eu/repository/handle/JRC97142
DOI: 10.3389/fchem.2016.00008
Type: Articles in periodicals and books
Abstract: In this work, Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS), Principal Component Analysis (PCA) and X-ray Photoelectron Spectroscopy (XPS) have been used to characterize the surface chemistry of gold substrates before and after functionalization with thiol-modified glucose self-assembled monolayers and subsequent biochemical specific recognition of maltose binding protein (MBP). The results indicate that the surface functionalization is achieved both on flat and nanoparticles gold substrates thus showing the potential of the developed system as biodetection platform. Moreover, the method presented here has been found to be a sound and valid approach to characterize the surface chemistry of nanoparticles functionalized with large molecules. Both techniques were proved to be very useful tools for monitoring all the functionalization steps, including the investigation of the biological behaviour of the glucose-modified particles in presence of the maltose binding protein.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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