Title: A systematic comparison of different techniques to determine the zeta potential of silica nanoparticles in biological medium
Authors: SIKORA AnetaBARTCZAK DorotaGEISSLER DanielKESTENS VikramROEBBEN GertRAMAYE YANNICVARGA Z.PALMAI MarcellSHARD AlexanderGOENAGA INFANTE HeidiMINELLI Caterina
Citation: ANALYTICAL METHODS no. 7 p. 9835–9843
Publisher: ROYAL SOC CHEMISTRY
Publication Year: 2015
JRC N°: JRC97261
ISSN: 1759-9660
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC97261
DOI: 10.1039/c5ay02014j
Type: Articles in periodicals and books
Abstract: The surface charge of nanoparticles plays an important role in the way they interact with biological systems. The ability to measure the surface charge of nanoparticles in biological media is therefore of importance in understanding the magnitude of such interactions. There are a number of methods which may be used to assess surface charge through the measurement of electrophoretic mobility. In order to better understand the comparability of these methods, the ζ-potential of silica nanoparticles in water, buffer and serum-based biological medium was measured by one ensemble and two particle-byparticle techniques: electrophoretic light scattering (ELS), tunable resistive pulse sensing (TRPS) and zeta particle tracking analysis (z-PTA). To allow comparability of results from different techniques, test samples were prepared according to an established protocol, although some variations were necessary to meet specific instrument requirements. Here we compare, for the first time, measurement results from the different techniques and discuss how modifications related to parameters such as environmental pH, dilution factor and presence of biomolecules influence the charge measurements.
JRC Directorate:Health, Consumers and Reference Materials

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