Please use this identifier to cite or link to this item:
|Title:||High variability of genomic instability and gene expression profiling in different HeLa clones|
|Authors:||FRATTINI Annalisa; FABBRI Marco; VALLI Roberto; DE PAOLI Elena; MONTALBANO Giuseppe; GRIBALDO Laura; PASQUALI Francesco; MASERATI Emanuela|
|Citation:||SCIENTIFIC REPORTS vol. 5 p. 15377|
|Publisher:||NATURE PUBLISHING GROUP|
|Type:||Articles in periodicals and books|
|Abstract:||The HeLa cell line is one of the most popular cell lines in biomedical research, despite its well-known chromosomal instability. We compared the genomic and transcriptomic profiles of 4 different HeLa batches and showed that the gain and loss of genomic material varies widely between batches, drastically affecting basal gene expression. Moreover, different pathways were activated in response to a hypoxic stimulus. Our study emphasizes the large genomic and transcriptomic variability among different batches, to the point that the same experiment performed with different batches can lead to distinct conclusions and irreproducible results. The HeLa cell line is thought to be a unique cell line but it is clear that substantial differences between the primary tumour and the human genome exist and that an indeterminate number of HeLa cell lines may exist, each with a unique genomic profile.|
|JRC Directorate:||Institute for Health and Consumer Protection Historical Collection|
Files in This Item:
There are no files associated with this item.
Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.