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dc.contributor.authorSCHNELL Sabineen_GB
dc.contributor.authorSTOTT Lucyen_GB
dc.contributor.authorHOGSTRAND Christeren_GB
dc.contributor.authorWOOD Chris Men_GB
dc.contributor.authorKELLY Scott Pen_GB
dc.contributor.authorPART Peteren_GB
dc.contributor.authorOWEN Stewart Fen_GB
dc.contributor.authorBURY Nic Ren_GB
dc.date.accessioned2016-03-20T01:08:31Z-
dc.date.available2016-03-18en_GB
dc.date.available2016-03-20T01:08:31Z-
dc.date.created2016-03-15en_GB
dc.date.issued2016en_GB
dc.date.submitted2015-12-17en_GB
dc.identifier.citationNATURE PROTOCOLS vol. 11 no. 3 p. 490–498en_GB
dc.identifier.issn1754-2189en_GB
dc.identifier.urihttp://www.nature.com/nprot/journal/v11/n3/full/nprot.2016.029.htmlen_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC99681-
dc.description.abstractThis protocol describes how to reconstruct and culture the freshwater rainbow trout gill epithelium on flat permeable membrane supports within cell culture inserts. The protocol describes gill cell isolation, cultured gill epithelium formation, maintenance, monitoring and preparation for use in experimental procedures. To produce a heterogeneous gill epithelium, as seen in vivo, seeding of isolated gill cells twice over a two day period is required. This is therefore termed the double seeded insert (DSI) technique. Approximately 5-12 days after cell isolation and seeding, preparations develop electrically tight gill epithelia that can withstand fresh water on the apical cell surface. The system can be used to study freshwater gill physiology, as well as a humane alternative for toxicity testing, bioaccumulation studies and environmental water quality monitoring.en_GB
dc.description.sponsorshipJRC.H.ADV01-Advisoren_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherNATURE PUBLISHING GROUPen_GB
dc.relation.ispartofseriesJRC99681en_GB
dc.titleProcedures for the reconstruction, primary culture and experimental use of rainbow trout gill epitheliaen_GB
dc.typeArticles in periodicals and booksen_GB
dc.identifier.doi10.1038/nprot.2016.029en_GB
JRC Directorate:Sustainable Resources

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