Proteomics for the safety assessment of nanomaterials: from the regulatory science perspective

Emerging approaches in the area of the safety assessment of nanomaterials combine the use of in vitro systems and post-genomics techniques. Our work should contribute to a better understanding of nanomaterials' effects at cellular level as well as propose alternative test methods to standardised regulatory methods when required1. The modification that occurs in the proteome profile of the human colon adenocarcinoma (Caco-2) cell line when exposed to nanosilver was investigated using two quantitative proteomic approaches. Both 2D gel-based and label-free MS-based proteomic approaches include extensive bioinformatics and data mining procedures. Confidence in protein identification and quantification, repeatability, instrument time, and cost are all important factors that need to be taken into account when comparing the two approaches. To date, however, no established guideline has yet been made available regarding the generation and assessment of the quality of proteomics data that could be reviewed by EU regulatory bodies for decision making. The aim of this work is to support and anticipate EU policy needs in the area of human exposure to nanomaterials by contributing to the development of new testing and predictive methods. References 1 Gioria S, Lobo Vicente J, Barboro P, La Spina R, Tomasi G, Urban P, Kinsner-Ovaskainen A, Rossi F, Chassaigne H. 2016. A combined proteomics and metabolomics approaches to assess the effect of gold nanoparticles in vitro. Nanotox. 10(16):736-748.

CHASSAIGNE Hubert;  HAJDUCH Martin;  BARBORO Paola;  URBAN LOPEZ Patricia;  GIORIA Sabrina; 

2016-11-18

Institute of Plant Genetics and Biotechnology & Biomedical Research Centre, Slovak Academy of Sciences

JRC103152

978-80-970662-3-9,   

http://www.inppo2016.sav.sk/data/book_of_abstracts.pdf,    https://publications.jrc.ec.europa.eu/repository/handle/JRC103152,