Determination of tropane alkaloids in cereals, tea and herbal infusions: Exploiting proficiency testing data as a basis to derive interlaboratory performance characteristics of an improved LC-MS/MS method
Monitoring of tropane alkaloids is regulated in the European Union in cereal-based foods for infants and young children, tea and herbal infusions. The European Commission’s Joint Research Centre (JRC) developed an improved LC-MS/MS analytical method using a pentafluorophenyl column, validated it and conducted two proficiency tests targeting these food categories. A subset of the data gathered from laboratories that used the JRC method was additionally exploited to derive interlaboratory performance characteristics.
The method showed fit-for-purpose figures of merit. The LOQs for atropine and scopolamine were around 0.4 and 1.2 μg/kg in cereal products, and in tea and herbal infusions, respectively. Uncertainties varied from 15 to 25%. The reproducibility varied from 11 to 38% for scopolamine and from 17 to 44% for atropine at levels ranging from 0.18 to 18.8 and 1.2–54.0 μg/kg, respectively. Recoveries ranged from 71 to 96%. These performance parameters render the method a good candidate for standardisation.
OLIVEIRA GONCALVES Carlos;
CUBERO LEON Elena;
STROKA Joerg;
2020-07-14
ELSEVIER SCI LTD
JRC117863
0308-8146 (online),
https://www.sciencedirect.com/science/article/pii/S0308814620311225?via%3Dihub,
https://publications.jrc.ec.europa.eu/repository/handle/JRC117863,
10.1016/j.foodchem.2020.127260 (online),
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