Validation of an Analytical Method for the Simultaneous Determination of Nine Intense Sweeteners by HPLC-ELSD
A collaborative trial was conducted to validate an analytical method for the simultaneous determination of nine intense sweeteners, i.e., acesulfame-K, alitame, aspartame, cyclamic acid, dulcin, neotame, neohesperidine dihydrochalcone, saccharin and sucralose in carbonated and non-carbonated soft drinks, and canned or bottled fruits. The procedure involves an extraction of the nine sweeteners with a buffer solution, sample clean-up using solid-phase extraction cartridges followed by an HPLC-ELSD analysis. Trueness, expressed in terms of recovery rates, was demonstrated in most cases by values ranging from 90 to 108%. High comparability of results obtained by individual testing laboratories was ensured by RSDR values <10% for the majority of results. Moreover, HorRAT values of less than 1.1 suggested for all sweeteners and matrices tested good performance of the method.
BUCHGRABER Manuela;
WASIK Andrzej;
2007-03-26
JRC36963
978-92-79-05354-2,
EUR 22726 EN,
OP LA-NA-22726-EN-C,
https://publications.jrc.ec.europa.eu/repository/handle/JRC36963,
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