Radiolabelling of TiO2 nanoparticles for radiotracer studies

Industrially manufactured titanium dioxide nanoparticles have been successfully radiolabelled with 48V by irradiation with a cyclotron-generated proton beam. Centrifugation tests showed that the 48V radio-labels were stably bound within the nanoparticle struc- ture, while X-ray di®raction indicated that no major structural modi¯cations to the nanoparticles resulted from the proton irradiation. In vitro tests of the uptake of cold and radiolabelled nanoparticles using the human cell line Calu-3 showed no signi¯cant di®erence in the uptake between both batches of nanoparticles. The uptake was quanti¯ed by Inductively Coupled Plasma Mass Spectrometry and high resolution °-ray spectrometry for cold and radiolabelled nanoparticles, respectively. These preliminary experiments indicate that al- terations to the nanoparticles' properties introduced by proton bombardment can be controlled to a su±cient extent to allow their use for meaningful biological in-vestigations as radiotracers.

ABBAS Kamel;  CYDZIK Izabel;  DEL TORCHIO Riccardo;  FARINA Massimo;  FORTI Efrat;  GIBSON Peter;  HOLZWARTH Uwe;  SIMONELLI Federica;  KREYLING Wolfgang; 

2010-10-19

SPRINGER

JRC52481

1388-0764,   

https://publications.jrc.ec.europa.eu/repository/handle/JRC52481,   

10.1007/s11051-009-9806-8,